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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat embryonic cerebral cortex Rat brain unknown plasmid Metafectene PRO application note

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown plasmid in vivo application Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Mouse immortalized skin fibroblasts Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

Mouse retinal ganglion cells (from multipotent retinal stem cells) Mouse sensory organs stem cell adherent plasmid K2 Transfection System publication D.-W. Chen et al., Prec. Nanomed., 2018, 1(2):106-123, DOI: 10.29016/180711.1 Link

Plutella xylostella larval hemocoel (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication L. Gong et al., Toxins, 2020, 12: 76, doi:10.3390/toxins12020076 Link

Human primary fibroblasts (derived from ALS patients) Human unknown cell line adherent siRNA RNA-Interference Metafectene publication M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1 Link

Spodoptera exigua (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Y. Park et al., Arch. Insect Biochem. Physiol., 2017, 95: e21386, oi.org/10.1002/arch.21386

Mouse articular chondrocytes Mouse other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication J. Rhee, J. of Biol. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014 Link

Mouse immortalized differentiated podocytes Mouse urinary system cell line adherent siRNA RNA-Interference K2 Transfection System publication J. G. Greiten et al., The FASEB Journal, 2021, 35: e21560, DOI: 10.1096/fj.202001179RR Link

Rat (Wistar adult male) hepatocytes Rat digestive organs unknown adherent plasmid DOTAP publication E. Skarpen et al., FASEB J, Feb 2008; 22: 466 - 476 Link

Plutella xylostella larvae (larval hemocoel) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication H. Antelmann et al., Original Res., 2020, doi: 10.3389/fmicb.2020.00528 Link

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent plasmid Metafectene publication Y. H. Huh et al., J. Biol. Chem., Jun 2007; 282: 17123 - 17131 Link

Spodoptera exigua larval hemocoel (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication B.-G. Choi et al., Comparative Biochem. and Physiol., 2014, Part A 168: 90-95, doi.org/10.1016/j.cbpa.2013.11.011

Spodoptera exigua third instar larvae (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J.-B. Lee et al., Arch. Insect Biochem. Physiol., 2017, 94: e21371, DOI: 10.1002/arch.21371

Mouse (BALB/c) primary alveolar macrophages Mouse immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Mouse articular chondrocytes Mouse unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2 Link

Maruca vitrata ( L4 or L5 larval hemocoel, legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. Al Baki et al., Pest Manag Sci, 2020; 76: 1020–1030, DOI 10.1002/ps.5612

Primary human skin fibroblasts Human skin primary cell adherent plasmid Metafectene publication T. Kiel, Dissertation 2012, Julius-Maximilians-Universität Würzburg

Plutella xylostella (hemocoels of third-instar NIL-R larvae, diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Z. Guo et al., PLoS Pathog., 2021, 17(9): e1009917, doi.org/10.1371/journal.ppat.1009917 Link

Spodoptera exigua (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Y. Park et al., Arch. Insect Biochem. Physiol., 2017, 95: e21386, oi.org/10.1002/arch.21386

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. M. I. Mollah et al., Front. Physiol., 2021, doi.org/10.3389/fphys.2021.744272 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA Metafectene PRO publication J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8

Spodoptera exigua Insect unknown unknown plasmid in vivo application Metafectene PRO publication S. Ahmed et al., J. of Exp. Biology, 2019, 222, doi:10.1242/jeb.207019 Link

Primary porcine granulosa cells Swine genital tract primary cell plasmid Metafectene PRO publication S-y Jung, Dissertation, 2020, Mississippi State University Link

Human primary fibroblasts Human unknown primary cell siRNA RNA-Interference Metafectene publication L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 Link

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