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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

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If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Spodoptera exigua third and fifth instar larvae (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication H. S. Kim et al., Journal of Asia-Pacific Entomology, March 2017, 20 (1): 199-205

Plutella xylostella larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Y. Park et al., Insect Molecular Biology, 2015, 24(6): 624-633; doi: 10.1111/imb.12188

Rat ventricular myocytes Rat muscle primary cell adherent plasmid RNA-Interference Metafectene publication B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26 Link

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Z. Wang et al., PLoS Genet., 2021, 17(9): e1009751. https://doi.org/10.1371/journal.pgen.1009751 Link

Human primary pterygium fibroblasts Human sensory organs primary cell adherent plasmid RNA-Interference Metafectene PRO publication Y. Su et al., Mol. Vis., 2011, 17: 247–256 Link

Aedes albopictus (mosquito, 5 days old females) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Md. A. Al Baki et al., bioRxiv, 2020, doi.org/10.1101/2020.07.17.208389 Link

Rat (newborn) ovary cells Rat genital tract unknown siRNA RNA-Interference Metafectene publication L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30

Plutella xylostella larvae (diamondback or cabbage moth) Insect unknown unknown siRNA RNA-Interference Metafectene PRO publication W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938 Link

Human primary skin fibroblasts Human skin primary cell adherent siRNA RNA-Interference Metafectene publication M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 Link

Tribolium castaneum (Hemocoel of late-instar larvae) Insect unknown unknown plasmid Metafectene PRO publication R. Hepat et al., J. Virol., October 2013, Volume 87, Number 20, 11223–11230 Link

Plutella xylostella hemocoel of early third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication J. Zhou et al., Pest Manag. Sci., 2020, 76: 712–720, DOI: 10.1002/ps.5569 Link

Plutella xylostella larval hemocoel (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication R. Ali et al., Comparative Biochem. and Physiol., 2015, Part A 179: 44-53; doi.org/10.1016/j.cbpa.2014.09.004

Rat primary skeletal myoblast cells (from male newborn Lewis rats) Rat muscle primary cell adherent plasmid Metafectene PRO publication B. Blumenthal et al., Eur. J. Cardio-thoracic Surg., 2011, 40: e135—e141, doi:10.1016/j.ejcts.2011.05.026 Link

Spodoptera exigua first-day fifth-instar larvae L5D1 (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication S. Shrestha et al., Archives of Insect Biochem. and Physiol., 2015, 89(4): 218-229; DOI: 10.1002/arch.21238

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Xenopus laevis (african clawed frog) intestinal fragments Frog digestive organs unknown plasmid Metafectene EASY application note

Spodoptera exigua larval haemocoel (3-day-old L4 larvae, beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. M. Sajjadian et al., Open Biol., 2020, 10: 200197, doi/10.1098/rsob.200197 Link

Mouse cultured hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Mouse primary embryonic forebrain cells Mouse brain primary cell plasmid Metafectene publication C. Karl et al., J Neurochemistry, 2005, 92, 264-282

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. A. Baki et al., Arch. Insect Biochem. Physiol., 2019:100:e21524, doi.org/10.1002/arch.21524

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. Vatanparast et al., Dev. and Comp. Immunology, 2019, 95: 108–117, doi.org/10.1016/j.dci.2019.02.008 Link

Maruca vitrata hemocoel of L5D1 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. A. Al Baki et al., PLoS ONE, 2018, 13 (10): e0204935, doi.org/10.1371/journal. pone.0204935 Link

Spodoptera exigua hemocoel first-day larvae L5 (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Y. Kim et al., Peptides, 2015, 68: 91-98; doi.org/10.1016/j.peptides.2015.02.003

Spodoptera exigua adult larvae (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Ahmed et al., Arch. Insect Biochem. Physiol.,2021, 106: e21748, doi.org/10.1002/arch.21748 Link

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