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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Rat primary granulosa cells isolated from Ovaries	Rat	genital tract	primary cell		plasmid		Metafectene	publication	J.-H. Kim et al., Nature Commun., 2014, 5:2936, DOI: 10.1038/ncomms3936	Link
Mouse primary embryonic forebrain cells	Mouse	brain	primary cell		plasmid		Metafectene	publication	C. Karl et al., J Neurochemistry, 2005, 92, 264-282
Human primary chronic lymphocytic leukemia cells	Human	immune system	primary cell	suspension	siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048	Link
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human fibrous dysplasia cells	Human	unknown	unknown		plasmid		Metafectene	publication	P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		oligonucleotide	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	E.-H. Hong et al., J. Biol. Chem., Aug 2011; 286: 28619 - 28631	Link
Rat sensory neurons isolated from dorsal root ganglia (DRG)	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8	Link
Mouse granulosa cells isolated from ICR mouse ovaries	Mouse	genital tract	primary cell		plasmid		Metafectene	publication	J.-H. Kim et al., J. Reprod. Dev., 2014, 60 (1) :14-20	Link
Mouse primary cortical cells	Mouse	brain	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340
Human fetal cortical stem cell cultures	Human	brain	stem cell	adherent	plasmid		Metafectene	publication	S. Couillard-Despres et al., 2014, US 8,841,430	Link
Rat (Sprague-Dawley) primary alveolar macrophages	Rat	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Hamster fetal ovary (organ culture)	Hamster	genital tract	organ		siRNA	RNA-Interference	Metafectene	publication	S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12
Mouse (18.5 dpc fetal or neonatal) ovaries	Mouse	genital tract	unknown		miRNA		Metafectene	publication	H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54	Link
Rabbit articular chondrocytes	Rabbit	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	J. Rhee, J. of Bio. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014	Link
Mouse fibroblast cell line, deficient of N-WASP	Mouse	unknown	cell line	adherent	plasmid		Metafectene	publication	S. Benesch et al., J. Cell Sci., Jul 2005; 118: 3103 - 3115	Link
Mouse (C57Bl/L6) cells in the area of the organ of Corti	Mouse	sensory organs	unknown		plasmid	in vivo application	Metafectene	publication	M. Praetorius et al., HNO, May 2008; 56(5): 524-9
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human Praeadipocytes	Human	skin	primary cell		plasmid		Metafectene	publication	G. Weisinger et al., US9663790B2	Link
Human primary fibroblasts	Human	unknown	primary cell		siRNA	RNA-Interference	Metafectene	publication	L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J. Rhee et al., J. Biol. Chem., Apr 2012; 287: 11751 - 11760	Link
Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries	Cow	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256	Link
Mouse cultured fetal ovaries	Mouse	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046	Link

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