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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	CAT.NO.	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Rat (Sprague Dawley young adult male) primary sensory neurons		Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748	Link
Rat primary skeletal myoblast cells (from male newborn Lewis rats)		Rat	muscle	primary cell	adherent	plasmid		Metafectene PRO	publication	B. Blumenthal et al., Eur. J. Cardio-thoracic Surg., 2011, 40: e135—e141, doi:10.1016/j.ejcts.2011.05.026	Link
Human primary chronic lymphocytic leukemia cells		Human	immune system	primary cell	suspension	siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048	Link
Human fibroblasts from CMT2B patients		Human	unknown	primary cell	adherent	plasmid		Metafectene PRO	publication	P. Saveri et al., Cells, 2020, 9, 1028, doi:10.3390/cells9041028	Link
Mouse primary myoblasts		Mouse	unknown	primary cell	adherent	miRNA		Metafectene PRO	publication	C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814	Link
Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats)		Rat	muscle	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113	Link
Mouse primary hepatocytes		Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212
Rat (Sprague Dawley young adult male) primary sensory neurons		Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529	Link
Mouse podocytes		Mouse	urinary system	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4	Link
Human Primary Peripheral Blood CD14+ Monocytes	ATCC PCS-800-010	Human	other	primary cell	suspension	miRNA		Metafectene SI	publication	S. Smith et al., Journal of Autoimmunity, 2017, 79, 105e111	Link
Mouse primary hepatocytes		Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	S. Surendran, PhD Thesis, 2014, Indiana University
Mouse primary hippocampal neurons		Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426	Link
Xenopus laevis (african clawed frog) primary intestine tissue		Frog	digestive organs	primary cell		plasmid		Metafectene EASY	publication	T. Hasebe et al., Developmental Dynamics 241:403–414, 2012
Human Praeadipocytes		Human	skin	primary cell		plasmid		Metafectene	publication	G. Weisinger et al., US9663790B2	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage		Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Rat (Sprague-Dawley) primary alveolar macrophages		Rat	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link
Human primary pterygium fibroblasts		Human	sensory organs	primary cell	adherent	plasmid	RNA-Interference	Metafectene PRO	publication	Y. Su et al., Mol. Vis., 2011, 17: 247–256	Link
Mouse primary hepatocytes		Mouse	digestive organs	primary cell	adherent	plasmid		Metafectene PRO	publication	J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8
Mouse hippocampal neurons		Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426	Link
Mouse primary hepatocytes		Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental
Mouse primary brown adipocyte		Mouse	other	primary cell		siRNA	RNA-Interference	K2 Transfection System	publication	J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374	Link
Mouse primary hepatocytes		Mouse	digestive organs	primary cell	adherent	miRNA	cotransfection (plasmid/miRNA)	Metafectene PRO	publication	S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958	Link
Mouse cultured cortical neurons		Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg
Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice		Mouse	brain	primary cell	adherent	siRNA		Metafectene PRO	publication	M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126	Link
Mouse articular chondrocytes		Mouse	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2	Link

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