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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA cotransfection (plasmid/miRNA) Metafectene PRO publication S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958 Link

Plutella xylostella early third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Z. Guo et al., 2015, PLoS Genet 11(4): e1005124. doi:10.1371/journal.pgen.1005124 Link

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown oligonucleotide in vivo application Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Mouse (18.5 dpc fetal or neonatal) ovaries Mouse genital tract unknown siRNA RNA-Interference Metafectene publication H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 Link

Mouse hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426 Link

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication A. Al Baki et al., Arch. Insect Biochem. Physiol., 2019, 100: e21524, DOI: 10.1002/arch.21524 Link

Spodoptera exigua larval hemocoel (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication S. M. Sajjadian et al., Arch. Insect Biochem. Physiol., 2019, 101: e21559, DOI: 10.1002/arch.21559 Link

Mouse chondrocytes Mouse other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828 Link

Xenopus laevis (african clawed frog) primary intestine tissue Frog digestive organs primary cell plasmid Metafectene EASY publication T. Hasebe et al., Developmental Dynamics 241:403–414, 2012

Spodoptera exigua larval hemocoel (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication S. Ahmed et al., J. of Experimental Biology, 2020, doi.org/10.1101/2020.07.13.201608

C57BL/6 mice bearing LLC Lewis lung carcinoma cells Mouse plasmid in vivo application Metafectene PRO publication V. Sankov et al., Prog. on Chem. and App. of Chitin and its Derivatives, 2017, 22: 190-200, DOI: 10.15259/PCACD.22.19 Link

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Ahmed et al., Front. Physiol., 2018, 9:1231, doi: 10.3389/fphys.2018.01231 Link

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication M. C. Roy et al., Front. Immunol., 2021, 2: 791319, doi: 10.3389/fimmu.2021.791319 Link

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication B. Park et al., Insect Science, 2012, 19: 47–54, DOI 10.1111/j.1744-7917.2011.01421.x

Leishmania donovani Protozoan unknown unknown plasmid K2 Transfection System application note

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown dsRNA cotransfection (plasmid/dsRNA) Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Spodoptera exigua larval hemocoel (L4) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication E. M. Sajjadian et al., Insect Mol. Biology, 2019, 28(6): 773–784, doi: 10.1111/imb.12588 Link

Human cutaneous melanoma (FFPE tissue sections) Human skin primary cell adherent plasmid Metafectene PRO publication M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004

Spodoptera exigua third instar larvae (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication J.-B. Lee et al., Arch. Insect Biochem. Physiol., 2017, 94: e21371, DOI: 10.1002/arch.21371

Mouse primary cortical cells Mouse brain primary cell adherent siRNA RNA-Interference Metafectene publication B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340

Plutella xylostella (1 day old L4 larvae, diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M.C. Roy et al., Arch. Insect Biochem. Physiol., 2020, 104: e21670, DOI: 10.1002/arch.21670

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878

Xenopus laevis XCT cells Frog unknown unknown plasmid RNA-Interference Metafectene PRO publication M. Dubinska-Magiera et al., Protoplasma, 2016, 253:943-956; DOI 10.1007/s00709-015-0861-y Link

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. C. Roy et al., J. Microbiol. Biotechnol., 2021, 31(4): 529–539, doi.org/10.4014/jmb.2012.12045 Link

Maruca vitrata female pupae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication W. H. Cha et al., Insect Sci., 2022, 0, 1–10, DOI 10.1111/1744-7917.12999 Link

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