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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats)	Rat	muscle	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113	Link
Mouse primary embryonic forebrain cells	Mouse	brain	primary cell		plasmid		Metafectene	publication	C. Karl et al., J Neurochemistry, 2005, 92, 264-282
Plutella xylostella hemocoel of early fourth instar larvae (diamondback or cabbage moth)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	M. R. Ali et al., Insect Molecular Biology, 2015, 24(1): 13-28; doi: 10.1111/imb.12132
Plutella xylostella, third instar larvae (diamondback or cabbage moth)	Insect	unknown	unknown		dsRNA	RNA-Interference	Metafectene PRO	publication	Z. Wang et al., PLoS Genet., 2021, 17(9): e1009751. https://doi.org/10.1371/journal.pgen.1009751	Link
Human primary pterygium fibroblasts	Human	sensory organs	primary cell	adherent	plasmid	RNA-Interference	Metafectene PRO	publication	Y. Su et al., Mol. Vis., 2011, 17: 247–256	Link
Primary porcine granulosa cells	Swine	genital tract	primary cell		plasmid		Metafectene PRO	publication	S-y Jung, Dissertation, 2020, Mississippi State University	Link
Spodoptera exigua fifth instar larvae (L5) (beet armyworm)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	J. Park et al., Archives of Insect Biochem. and Physiol., 2014, 85(4) :234-247, DOI: 10.1002/arch.21156
Rat primary hepatocytes (Sprague Dawley rats)	Rat	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991	Link
Plodia interpunctella fifth-instar larva (Indian meal moth)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	M. Kim et al., Environmental Entomology, 2017, 46 (4): 1005-1011, doi: 10.1093/ee/nvx112
Mouse hippocampal neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426	Link
Spodoptera exigua forth and fifth instar larvae (beet armyworm)	Insect	unknown	unknown		dsRNA	RNA-Interference	Metafectene PRO	publication	S. Ahmed et al., J. of Invertebrate Pathology, 2018, doi.org/10.1016/j.jip.2018.05.009
Rat (Wistar adult male) hepatocytes	Rat	digestive organs	unknown	adherent	plasmid		DOTAP	publication	E. Skarpen et al., FASEB J, Feb 2008; 22: 466 - 476	Link
Spodoptera exigua (beet armyworm) L5 larvae	Insect	unknown	unknown		dsRNA	RNA-Interference	Metafectene PRO	publication	M.Sadekuzzaman et al., PLOSONE, 2018; doi.org/10.1371/journal.pone.0193282	Link
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human primary CD14+ monocytes	Human	other	primary cell	suspension	miRNA		Metafectene SI	publication	S. Smith, JCI Insight, 2018, 3(15):e120798, doi.org/10.1172/jci.insight.120798	Link
Plutella xylostella larvae (diamondback or cabbage moth)	Insect	unknown	unknown		siRNA	RNA-Interference	Metafectene PRO	publication	W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938	Link
Rat primary skeletal myoblast cells (from male newborn Lewis rats)	Rat	muscle	primary cell	adherent	plasmid		Metafectene PRO	publication	B. Blumenthal et al., Eur. J. Cardio-thoracic Surg., 2011, 40: e135—e141, doi:10.1016/j.ejcts.2011.05.026	Link
Leishmania donovani promastigotes	Protozoan	unknown	unknown		plasmid		K2 Transfection System	publication	I. S. Chauhan et al., Acta Tropica, 2019, 199, 105158, doi.org/10.1016/j.actatropica.2019.105158	Link
Hyphantria cunea pupae (fall webworm)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	F. Yang et al., Global J. of Advanced Research, 2016, 3(11): 985-991	Link
Plodia interpunctella larvae (Indian meal moth)	Insect	unknown	unknown		dsRNA	RNA-Interference	Metafectene PRO	publication	G. Deok Han et al., Pesticide Biochem. and Physiol., Nov. 2017, 143: 48-56, doi.org/10.1016/j.pestbp.2017.09.010
Spodoptera exigua larvae (L5) (beet armyworm)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	M. C. Roy et al., Front. Immunol., 2021, 2: 791319, doi: 10.3389/fimmu.2021.791319	Link
Spodoptera exigua adult larvae (beet armyworm)	Insect	unknown	unknown		dsRNA	RNA-Interference	Metafectene PRO	publication	S. Ahmed et al., Arch. Insect Biochem. Physiol.,2021, 106: e21748, doi.org/10.1002/arch.21748	Link
Plutella xylostella, third instar larvae (diamondback or cabbage moth)	Insect	unknown	unknown		plasmid	in vivo application	Metafectene PRO	publication	B. Park et al., Insect Science, 2012, 19: 47–54, DOI 10.1111/j.1744-7917.2011.01421.x
Plutella xylostella early third instar larvae (diamondback or cabbage moth)	Insect	unknown	unknown		dsRNA	in vivo application	Metafectene PRO	publication	Z. Guo et al., Scientific Reports, 2015, 5:13728; DOI: 10.1038/srep13728	Link
Mouse (BALB/c) primary alveolar macrophages	Mouse	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link

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