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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Mouse (18.5 dpc fetal or neonatal) ovaries	Mouse	genital tract	unknown		miRNA		Metafectene	publication	H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Hamster fetal ovary (organ culture)	Hamster	genital tract	organ		siRNA	RNA-Interference	Metafectene	publication	S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12
Rabbit articular chondrocytes	Rabbit	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	J. Rhee, J. of Bio. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014	Link
Mouse fibroblast cell line, deficient of N-WASP	Mouse	unknown	cell line	adherent	plasmid		Metafectene	publication	S. Benesch et al., J. Cell Sci., Jul 2005; 118: 3103 - 3115	Link
Mouse (C57Bl/L6) cells in the area of the organ of Corti	Mouse	sensory organs	unknown		plasmid	in vivo application	Metafectene	publication	M. Praetorius et al., HNO, May 2008; 56(5): 524-9
Human primary fibroblasts	Human	unknown	primary cell		siRNA	RNA-Interference	Metafectene	publication	L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997	Link
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human Praeadipocytes	Human	skin	primary cell		plasmid		Metafectene	publication	G. Weisinger et al., US9663790B2	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J. Rhee et al., J. Biol. Chem., Apr 2012; 287: 11751 - 11760	Link
Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries	Cow	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256	Link
Mouse cultured fetal ovaries	Mouse	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046	Link
Mouse chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828	Link
Human tenon’s capsule fibroblasts	Human	sensory organs	unknown		oligonucleotide		Metafectene	publication	H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290
Rat primary pinealocyte cells	Rat	brain	primary cell		plasmid		Metafectene	publication	T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810	Link
Rat (Wistar neonatal) primary hippocampal neurons	Rat	brain	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567	Link
Mouse (18.5 dpc fetal or neonatal) ovaries	Mouse	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54	Link
Rat ventricular myocytes	Rat	muscle	primary cell	adherent	plasmid	RNA-Interference	Metafectene	publication	B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	Y. H. Huh et al., J. Biol. Chem., Jun 2007; 282: 17123 - 17131	Link
Mouse articular chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	J. Rhee, J. of Biol. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014	Link
Rat (Sprague-Dawley)	Rat	unknown	unknown		plasmid	in vivo application	Metafectene	publication	T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99
Primary human skin fibroblasts	Human	skin	primary cell	adherent	plasmid		Metafectene	publication	T. Kiel, Dissertation 2012, Julius-Maximilians-Universität Würzburg
Human primary skin fibroblasts	Human	skin	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1	Link
Rat (newborn) ovary cells	Rat	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30
Mouse (nude) xenografted tumor, established by human Hep-2 cells	Mouse	unknown	unknown		plasmid	RNA-Interference	Metafectene	publication	J. Zhou et al., Lin Chuang Er Bi Yan Hou Ke Za Zhi, Mar 2006; 20(6): 264-7

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