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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8
Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries	Cow	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256	Link
Mouse cultured hippocampal neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	miRNA		Metafectene PRO	publication	C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	plasmid	cotransfection (plasmid/miRNA)	Metafectene PRO	publication	S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958	Link
Human peripheral blood mononuclear cell (PBMC)-derived macrophages	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8	Link
Human uveal melanoma (FFPE tissue sections)	Human	skin	primary cell	adherent	plasmid		Metafectene PRO	publication	M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004
Mouse fetal ovaries	Mouse	genital tract	primary cell		siRNA	RNA-Interference	Metafectene PRO	publication	R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R.	Link
Mouse embryonic forebrain cultures	Mouse	brain	primary cell	adherent	plasmid		Metafectene	publication	S. Couillard-Despres et al., 2014, US 8,841,430	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878
Rat (Wistar embryonic) primary hippocampal neurons	Rat	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	K. Kankowski, Dissertation, 2015, Universitätsklinikum Hamburg-Eppendorf	Link
Mouse primary cortical cells	Mouse	brain	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340
Human primary fibroblasts	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360	Link
Human primary hepatocytes	Human	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	E.-H. Hong et al., J. Biol. Chem., Aug 2011; 286: 28619 - 28631	Link
Mouse chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828	Link
Human fibroblasts from CMT2B patients	Human	unknown	primary cell	adherent	plasmid		Metafectene PRO	publication	P. Saveri et al., Cells, 2020, 9, 1028, doi:10.3390/cells9041028	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748	Link
Rat primary skeletal myoblast cells (from male newborn Lewis rats)	Rat	muscle	primary cell	adherent	plasmid		Metafectene PRO	publication	B. Blumenthal et al., Eur. J. Cardio-thoracic Surg., 2011, 40: e135—e141, doi:10.1016/j.ejcts.2011.05.026	Link
Human primary chronic lymphocytic leukemia cells	Human	immune system	primary cell	suspension	siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048	Link
Mouse primary myoblasts	Mouse	unknown	primary cell	adherent	miRNA		Metafectene PRO	publication	C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814	Link
Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats)	Rat	muscle	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212
Mouse podocytes	Mouse	urinary system	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4	Link

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