References
Products Transfection
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
| CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chicken cerebellar granule neurons | Chicken | brain | primary cell | adherent | plasmid | Metafectene PRO | publication | L. P. E. Austdal et al., Journal of Neuroendocrinology, 2016, 28, doi: 10.1111/jne.1243 | |||||
| Rat primary hepatocytes (Sprague Dawley rats) | Rat | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991 | Link | |||
| Rat (Sprague Dawley young adult male) primary sensory neurons | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 | Link | |||
| Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) | Rat | muscle | primary cell | adherent | plasmid | K2 Transfection System | publication | E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 | Link | ||||
| Maruca vitrata late L4 larvae (legume pod borer) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | A. Al Baki et al., Pest Manag. Sci., 2020, 76: 1020–1030, DOI: 10.1002/ps.5612 | Link | ||||
| Plutella xylostella larvae (diamondback or cabbage moth) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | Y. Park et al., Insect Molecular Biology, 2015, 24(6): 624-633; doi: 10.1111/imb.12188 | |||||
| Plutella xylostella, third instar larvae (diamondback or cabbage moth) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | Z. Wang et al., PLoS Genet., 2021, 17(9): e1009751. https://doi.org/10.1371/journal.pgen.1009751 | Link | ||||
| Rat primary hepatocytes | Rat | digestive organs | primary cell | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906 | ||||
| Rat sensory neurons isolated from dorsal root ganglia (DRG) | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 | Link | |||
| 4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein | Mouse | unknown | unknown | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 | Link | ||||
| Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood | Human | other | stem cell | adherent | plasmid | K2 Transfection System | publication | S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681 | |||||
| Mouse primary brown adipocyte | Mouse | other | primary cell | siRNA | RNA-Interference | K2 Transfection System | publication | J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374 | Link | ||||
| Maruca vitrata hemocoel of L5 larvae (legume pod borer) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | A. Al Baki et al., Arch. Insect Biochem. Physiol., 2019, 100: e21524, DOI: 10.1002/arch.21524 | Link | ||||
| Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice | Mouse | brain | primary cell | adherent | siRNA | Metafectene PRO | publication | M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126 | Link | ||||
| Human tenon’s capsule fibroblasts | Human | sensory organs | unknown | oligonucleotide | Metafectene | publication | H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290 | ||||||
| Mouse fetal ovaries | Mouse | genital tract | primary cell | siRNA | RNA-Interference | Metafectene PRO | publication | R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R. | Link | ||||
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | miRNA | Metafectene PRO | publication | C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental | |||||
| Maruca vitrata hemocoel of L5 larvae (legume pod borer) | Insect | unknown | unknown | dsRNA | in vivo application | Metafectene PRO | publication | A. A. Baki et al., Arch. Insect Biochem. Physiol., 2019:100:e21524, doi.org/10.1002/arch.21524 | |||||
| Spodoptera exigua larvae (L5) (beet armyworm) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | M. Vatanparast et al., Dev. and Comp. Immunology, 2019, 95: 108–117, doi.org/10.1016/j.dci.2019.02.008 | Link | ||||
| Spodoptera exigua fifth instar larvae (L5) (beet armyworm) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | S. Ahmed et al., Front. Physiol., 2018, 9:1231, doi: 10.3389/fphys.2018.01231 | Link | ||||
| Human primary chronic lymphocytic leukemia cells | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link | |||
| Spodoptera exigua larvae (L5) (beet armyworm) | Insect | unknown | unknown | dsRNA | in vivo application | Metafectene PRO | publication | M. C. Roy et al., Front. Immunol., 2021, 2: 791319, doi: 10.3389/fimmu.2021.791319 | Link | ||||
| Spodoptera exigua adult larvae (beet armyworm) | Insect | unknown | unknown | dsRNA | RNA-Interference | Metafectene PRO | publication | S. Ahmed et al., Arch. Insect Biochem. Physiol.,2021, 106: e21748, doi.org/10.1002/arch.21748 | Link | ||||
| Hamster fetal ovary (organ culture) | Hamster | genital tract | organ | siRNA | RNA-Interference | Metafectene | publication | S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12 | |||||
| Mouse cortical neuronal cells | Mouse | brain | unknown | adherent | plasmid | Metafectene EASY | application note |  |
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