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Products Transfection
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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878

Xenopus laevis XCT cells Frog unknown unknown plasmid RNA-Interference Metafectene PRO publication M. Dubinska-Magiera et al., Protoplasma, 2016, 253:943-956; DOI 10.1007/s00709-015-0861-y Link

Spodoptera exigua larval hemocoel (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. Mahi Imam Mollah et al., PLoS Pathog., 2021, 17(3): e1009467, doi.org/10.1371/journal.ppat.1009467 Link

Human primary hepatocytes Human digestive organs primary cell adherent miRNA Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Plutella xylostella hemocoel of L3D1 NP larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Kumar et al., J. of General Virology, 2016, 97: 2780-2796; DOI 10.1099/jgv.0.000560 Link

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown dsRNA cotransfection (plasmid/dsRNA) Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Human cutaneous melanoma (FFPE tissue sections) Human skin primary cell adherent plasmid Metafectene PRO publication M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication M. T. H. Hrithik et al., Insects, 2021, 12,449, doi.org/10.3390/insects12050449 Link

Spodoptera exigua third instar larvae (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication J.-B. Lee et al., Arch. Insect Biochem. Physiol., 2017, 94: e21371, DOI: 10.1002/arch.21371

Mouse articular chondrocytes Mouse unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2 Link

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J. Park et al., PLoS ONE, 2014, 9(9): e105717, doi:10.1371/journal.pone.0105717 Link

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent plasmid K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Mouse fibroblast cell line, deficient of N-WASP Mouse unknown cell line adherent plasmid Metafectene publication S. Benesch et al., J. Cell Sci., Jul 2005; 118: 3103 - 3115 Link

Plutella xylostella hemocoel of NP3 larvae (diamondback or cabbage moth) Insect unknown unknown plasmid in vivo application Metafectene PRO publication S. Kumar et al., PLOSONE, 2017, doi.org/10.1371/journal.pone.0177066 Link

Primary human skin fibroblasts Human skin primary cell adherent plasmid Metafectene publication T. Kiel, Dissertation 2012, Julius-Maximilians-Universität Würzburg

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication Z. Wang et al., PLoS Genet., 2021, 17(9): e1009751. https://doi.org/10.1371/journal.pgen.1009751 Link

Spodoptera exigua (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Y. Park et al.,BMC Biology, 2014, 12: 46, doi:10.1186/1741-7007-12-46 Link

Mouse cultured fetal ovaries Mouse genital tract primary cell siRNA RNA-Interference Metafectene publication B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046 Link

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication J. Park et al., Archives of Insect Biochem. and Physiol., 2014, 85(4) :234-247, DOI: 10.1002/arch.21156

Human primary skin fibroblasts Human skin primary cell adherent siRNA RNA-Interference Metafectene publication M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 Link

Plutella xylostella hemocoel of early third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Kumar et al., Scientific Reports, 7:43287, DOI: 10.1038/srep43287 Link

Antheraea pernyi pupae in diaupase (Chinese Tasar Moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. A. M. Mohamed et al., PLoS ONE, 2014, 9(3): e92680, doi:10.1371/journal.pone.0092680 Link

Spodoptera exigua forth and fifth instar larvae (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication S. Ahmed et al., J. of Invertebrate Pathology, 2018, doi.org/10.1016/j.jip.2018.05.009

Maruca vitrata late L4 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication A. Al Baki et al., Pest Manag. Sci., 2020, 76: 1020–1030, DOI: 10.1002/ps.5612 Link

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