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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Mouse chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828	Link
Rat primary neonatal ventricular cardiomyocytes	Rat	muscle	primary cell		plasmid		K2 Transfection System	application note
Human fibrous dysplasia cells	Human	unknown	unknown		plasmid		Metafectene	publication	P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44
Human Praeadipocytes	Human	skin	primary cell		plasmid		Metafectene	publication	G. Weisinger et al., US9663790B2	Link
Human primary hepatocytes	Human	digestive organs	primary cell	adherent	plasmid	RNA-Interference	Metafectene PRO	publication	J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8	Link
Human fibroblasts from CMT2B patients	Human	unknown	primary cell	adherent	plasmid		Metafectene PRO	publication	P. Saveri et al., Cells, 2020, 9, 1028, doi:10.3390/cells9041028	Link
Mouse immortalized skin fibroblasts (deficient of Lap2-alpha)	Mouse	unknown	unknown	adherent	plasmid		Metafectene PRO	publication	E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1
Plutella xylostella hemocoel of late second instar larvae	Insect	unknown	unknown		plasmid	RNA-Interference	Metafectene PRO	publication	S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017
Chicken cerebellar granule neurons	Chicken	brain	unknown	adherent	plasmid		Metafectene PRO	publication	B. O. Strom et al., J Neurosci Methods, Oct 2010; 193(1): 39-46
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood	Human	other	stem cell	adherent	plasmid		K2 Transfection System	publication	S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681
Mouse mixed neuron-glia cerebellar cultures	Mouse	brain	unknown	adherent	plasmid	cotransfection (plasmid/plasmid)	K2 Transfection System	application note
Rat (Sprague-Dawley)	Rat	unknown	unknown		plasmid	in vivo application	Metafectene	publication	T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99
Mouse primary hippocampal neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426	Link
Spodoptera exigua hemocytes of late fourth instar larvae	Insect	unknown	unknown		plasmid	in vivo application	Metafectene PRO	application note
Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats)	Rat	muscle	primary cell	adherent	plasmid		K2 Transfection System	publication	E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113	Link
Plutella xylostella larval hemocoel (diamondback or cabbage moth)	Insect	unknown	unknown		plasmid		Metafectene PRO	publication	R. Ali et al., Comparative Biochem. and Physiol., 2015, Part A 179: 44-53; doi.org/10.1016/j.cbpa.2014.09.004
Human cutaneous melanoma (FFPE tissue sections)	Human	skin	primary cell	adherent	plasmid		Metafectene PRO	publication	M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004
Rat primary hepatocytes	Rat	digestive organs	primary cell	adherent	plasmid	cotransfection (plasmid/siRNA)	Metafectene PRO	publication	F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906
Rat primary pinealocyte cells	Rat	brain	primary cell		plasmid		Metafectene	publication	T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810	Link
Neurosphere derived neuroprogenitor cells (isolated from newborn mice brains)	Mouse	brain	unknown	adherent	plasmid	cotransfection (plasmid/plasmid)	K2 Transfection System	publication	S.Y. Kim et al., J. of Cell Science, 2019, 132, jcs228940, doi:10.1242/jcs.228940	Link
Mouse (BALB/c) primary alveolar macrophages	Mouse	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link
Mouse cultured cortical neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg
Mouse embryonic forebrain cultures	Mouse	brain	primary cell	adherent	plasmid		Metafectene	publication	S. Couillard-Despres et al., 2014, US 8,841,430	Link
Mouse hepatocytes	Mouse	digestive organs	primary cell	adherent	plasmid		Metafectene PRO	publication	S.-Y. Cai et al., JCI Insight. 2017, 2(5): e90780, doi.org/10.1172/jci.insight.90780