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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown oligonucleotide in vivo application Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Mouse (18.5 dpc fetal or neonatal) ovaries Mouse genital tract unknown siRNA RNA-Interference Metafectene publication H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 Link

Mouse hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication S.-Y. Cai et al., JCI Insight. 2017, 2(5): e90780, doi.org/10.1172/jci.insight.90780

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Surendran, PhD Thesis, 2014, Indiana University

Mouse cortical neuronal cells Mouse brain unknown adherent plasmid Metafectene EASY application note

Mouse podocytes Mouse urinary system primary cell adherent siRNA RNA-Interference K2 Transfection System publication F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4 Link

Mouse cultured hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Mouse (BALB/c mice) organ of Corti explants Mouse sensory organs unknown miRNA K2 Transfection System publication Y. Li et al., Cell Death and Disease, 2016, 7:e2362, doi:10.1038/cddis.2016.246 Link

Mouse primary embryonic forebrain cells Mouse brain primary cell plasmid Metafectene publication C. Karl et al., J Neurochemistry, 2005, 92, 264-282

Mouse (BALB/c) primary alveolar macrophages Mouse immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Mouse granulosa cells isolated from ICR mouse ovaries Mouse genital tract primary cell plasmid Metafectene publication J.-H. Kim et al., J. Reprod. Dev., 2014, 60 (1) :14-20 Link

Mouse primary myoblasts Mouse unknown primary cell adherent miRNA Metafectene PRO publication C. E. Winbanks et al., J. Biol. Chem., Apr 2011; 286: 13805 - 13814 Link

Mouse immortalized skin fibroblasts (deficient of Lap2-alpha) Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid cotransfection (plasmid/miRNA) Metafectene PRO publication S. Surendran et al., Scientific Reports, 2016, 6:18958; DOI: 10.1038/srep18958 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene PRO publication S. Surendran, PhD Thesis, 2014, Indiana University

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212

Mouse fetal ovaries Mouse genital tract primary cell siRNA RNA-Interference Metafectene PRO publication R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R. Link

Mouse chondrocytes Mouse other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828 Link

Mouse articular chondrocytes Mouse unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2 Link

Mouse primary cortical cells Mouse brain primary cell adherent siRNA RNA-Interference Metafectene publication B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340

Mouse primary hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878

Mouse hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426 Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8

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