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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Human peripheral blood mononuclear cell (PBMC)-derived macrophages	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748	Link
Mouse primary brown adipocyte	Mouse	other	primary cell		siRNA	RNA-Interference	K2 Transfection System	publication	J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374	Link
Human primary fibroblasts	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360	Link
Mouse articular chondrocytes	Mouse	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2	Link
Hamster fetal ovary (organ culture)	Hamster	genital tract	organ		siRNA	RNA-Interference	Metafectene	publication	S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12
Rat primary hepatocytes (Sprague Dawley rats)	Rat	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991	Link
Mouse immortalized differentiated podocytes	Mouse	urinary system	cell line	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	J. G. Greiten et al., The FASEB Journal, 2021, 35: e21560, DOI: 10.1096/fj.202001179RR	Link
Human primary hepatocytes	Human	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8	Link
Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries	Cow	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256	Link
Plutella xylostella larvae (diamondback or cabbage moth)	Insect	unknown	unknown		siRNA	in vivo application	Metafectene PRO	publication	W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335	Link
Plutella xylostella larvae (diamondback or cabbage moth)	Insect	unknown	unknown		siRNA	RNA-Interference	Metafectene PRO	publication	W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938	Link
Human keratinocytes isolated from neonatal foreskin	Human	skin	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	S. Bruche, PhD Thesis, 2014, Imperial College London	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878
Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice	Mouse	brain	primary cell	adherent	siRNA		Metafectene PRO	publication	M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126	Link
Rat sensory neurons isolated from dorsal root ganglia (DRG)	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Rat primary hepatocytes	Rat	digestive organs	primary cell	adherent	siRNA	cotransfection (plasmid/siRNA)	Metafectene PRO	publication	F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906
Human primary skin fibroblasts	Human	skin	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1	Link
Mouse cultured fetal ovaries	Mouse	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link

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