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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Rat (Wistar neonatal) primary hippocampal neurons	Rat	brain	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567	Link
Rat ventricular myocytes	Rat	muscle	primary cell	adherent	plasmid	RNA-Interference	Metafectene	publication	B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	Y. H. Huh et al., J. Biol. Chem., Jun 2007; 282: 17123 - 17131	Link
Mouse (18.5 dpc fetal or neonatal) ovaries	Mouse	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54	Link
Mouse articular chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	J. Rhee, J. of Biol. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014	Link
Rat (Sprague-Dawley)	Rat	unknown	unknown		plasmid	in vivo application	Metafectene	publication	T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99
Human primary skin fibroblasts	Human	skin	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1	Link
Rat (newborn) ovary cells	Rat	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30
Primary human skin fibroblasts	Human	skin	primary cell	adherent	plasmid		Metafectene	publication	T. Kiel, Dissertation 2012, Julius-Maximilians-Universität Würzburg
Mouse (nude) xenografted tumor, established by human Hep-2 cells	Mouse	unknown	unknown		plasmid	RNA-Interference	Metafectene	publication	J. Zhou et al., Lin Chuang Er Bi Yan Hou Ke Za Zhi, Mar 2006; 20(6): 264-7
Mouse embryonic forebrain cultures	Mouse	brain	primary cell	adherent	plasmid		Metafectene	publication	S. Couillard-Despres et al., 2014, US 8,841,430	Link
Mouse (BALB/c) primary alveolar macrophages	Mouse	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link
Human keratinocytes isolated from neonatal foreskin	Human	skin	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	S. Bruche, PhD Thesis, 2014, Imperial College London	Link
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335	Link
Human primary fibroblasts (derived from ALS patients)	Human	unknown	cell line	adherent	siRNA	RNA-Interference	Metafectene	publication	M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1	Link
Rabitt (Lapine) articular chondrocytes	Rabbit	other	unknown		plasmid		Metafectene	publication	P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44
Primary rat neuronal dendrites	Rat	nervous system	primary cell	adherent	mRNA		Metafectene	publication	J. Eberwine et al., 2013, US 8,367.402
Rat primary granulosa cells isolated from Ovaries	Rat	genital tract	primary cell		plasmid		Metafectene	publication	J.-H. Kim et al., Nature Commun., 2014, 5:2936, DOI: 10.1038/ncomms3936	Link
Mouse primary embryonic forebrain cells	Mouse	brain	primary cell		plasmid		Metafectene	publication	C. Karl et al., J Neurochemistry, 2005, 92, 264-282
Human primary chronic lymphocytic leukemia cells	Human	immune system	primary cell	suspension	siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048	Link
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human fibrous dysplasia cells	Human	unknown	unknown		plasmid		Metafectene	publication	P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		oligonucleotide	in vivo application	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link

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