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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529	Link
Mouse (18.5 dpc fetal or neonatal) ovaries	Mouse	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54	Link
4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein	Mouse	unknown	unknown		siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109	Link
Human primary chronic lymphocytic leukemia cells	Human	immune system	primary cell	suspension	siRNA	RNA-Interference	Metafectene	publication	S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048	Link
Human immortalized lymphocyte cell line (immortalized by EBV)	Human	immune system	cell line	suspension	siRNA	RNA-Interference	Metafectene PRO	publication	M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065	Link
Mouse podocytes	Mouse	urinary system	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4	Link
Mouse primary cortical cells	Mouse	brain	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340
Human primary fibroblasts	Human	unknown	primary cell		siRNA	RNA-Interference	Metafectene	publication	L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997	Link
Mouse fetal ovaries	Mouse	genital tract	primary cell		siRNA	RNA-Interference	Metafectene PRO	publication	R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R.	Link
Human primary fibroblasts (derived from ALS patients)	Human	unknown	cell line	adherent	siRNA	RNA-Interference	Metafectene	publication	M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1	Link
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	S. Surendran, PhD Thesis, 2014, Indiana University
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	E.-H. Hong et al., J. Biol. Chem., Aug 2011; 286: 28619 - 28631	Link
Rat (newborn) ovary cells	Rat	genital tract	unknown		siRNA	RNA-Interference	Metafectene	publication	L. Q. Xu et al., Sheng Li Xue Bao, Oct 2009; 61(5): 424-30
Human peripheral blood mononuclear cell (PBMC)-derived macrophages	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	X. X. Chi et al., J Neurophysiol, Nov 2010; 104: 2741 - 2748	Link
Mouse primary brown adipocyte	Mouse	other	primary cell		siRNA	RNA-Interference	K2 Transfection System	publication	J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374	Link
Human primary fibroblasts	Human	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360	Link
Mouse articular chondrocytes	Mouse	unknown	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	S. Kim et al., Nature Communications, 2019, 10:4898, doi.org/10.1038/s41467-019-12910-2	Link
Hamster fetal ovary (organ culture)	Hamster	genital tract	organ		siRNA	RNA-Interference	Metafectene	publication	S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12
Rat primary hepatocytes (Sprague Dawley rats)	Rat	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991	Link
Mouse immortalized differentiated podocytes	Mouse	urinary system	cell line	adherent	siRNA	RNA-Interference	K2 Transfection System	publication	J. G. Greiten et al., The FASEB Journal, 2021, 35: e21560, DOI: 10.1096/fj.202001179RR	Link
Human primary hepatocytes	Human	digestive organs	primary cell	adherent	siRNA	RNA-Interference	Metafectene PRO	publication	J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8	Link
Bovine granulosa cells isolated from follicles (2-8 mm in diameter) of bovine ovaries	Cow	genital tract	primary cell		siRNA	RNA-Interference	Metafectene	publication	M. R. Plewes et al., bioRxiv, 2018, doi.org/10.1101/406256	Link
Plutella xylostella larvae (diamondback or cabbage moth)	Insect	unknown	unknown		siRNA	in vivo application	Metafectene PRO	publication	W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938	Link
Rat (Sprague Dawley young adult male) primary sensory neurons	Rat	nervous system	primary cell	adherent	siRNA	RNA-Interference	Metafectene	publication	Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335	Link

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