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Products Transfection
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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Mouse chondrocytes Mouse other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828 Link

Rat primary neonatal ventricular cardiomyocytes Rat muscle primary cell plasmid K2 Transfection System application note

Human fibrous dysplasia cells Human unknown unknown plasmid Metafectene publication P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44

Human Praeadipocytes Human skin primary cell plasmid Metafectene publication G. Weisinger et al., US9663790B2 Link

Human primary hepatocytes Human digestive organs primary cell adherent plasmid RNA-Interference Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Human fibroblasts from CMT2B patients Human unknown primary cell adherent plasmid Metafectene PRO publication P. Saveri et al., Cells, 2020, 9, 1028, doi:10.3390/cells9041028 Link

Mouse immortalized skin fibroblasts (deficient of Lap2-alpha) Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown plasmid RNA-Interference Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Chicken cerebellar granule neurons Chicken brain unknown adherent plasmid Metafectene PRO publication B. O. Strom et al., J Neurosci Methods, Oct 2010; 193(1): 39-46

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent plasmid Metafectene publication J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047 Link

Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood Human other stem cell adherent plasmid K2 Transfection System publication S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681

Mouse mixed neuron-glia cerebellar cultures Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

Rat (Sprague-Dawley) Rat unknown unknown plasmid in vivo application Metafectene publication T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99

Mouse primary hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426 Link

Spodoptera exigua hemocytes of late fourth instar larvae Insect unknown unknown plasmid in vivo application Metafectene PRO application note

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent plasmid K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Plutella xylostella larval hemocoel (diamondback or cabbage moth) Insect unknown unknown plasmid Metafectene PRO publication R. Ali et al., Comparative Biochem. and Physiol., 2015, Part A 179: 44-53; doi.org/10.1016/j.cbpa.2014.09.004

Human cutaneous melanoma (FFPE tissue sections) Human skin primary cell adherent plasmid Metafectene PRO publication M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004

Rat primary hepatocytes Rat digestive organs primary cell adherent plasmid cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Rat primary pinealocyte cells Rat brain primary cell plasmid Metafectene publication T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810 Link

Neurosphere derived neuroprogenitor cells (isolated from newborn mice brains) Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication S.Y. Kim et al., J. of Cell Science, 2019, 132, jcs228940, doi:10.1242/jcs.228940 Link

Mouse (BALB/c) primary alveolar macrophages Mouse immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Mouse cultured cortical neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg

Mouse embryonic forebrain cultures Mouse brain primary cell adherent plasmid Metafectene publication S. Couillard-Despres et al., 2014, US 8,841,430 Link

Mouse hepatocytes Mouse digestive organs primary cell adherent plasmid Metafectene PRO publication S.-Y. Cai et al., JCI Insight. 2017, 2(5): e90780, doi.org/10.1172/jci.insight.90780

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