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References

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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.

DESCRIPTION OF CELL TYPE	SPECIES	ORIGIN OF CELLS	COMMON CELL TYPE	GROWTH PROPERTIES	NUCLEIC ACID / DELIVERED MOLECULE	APPLICATION	PRODUCT	REFERENCE TYPE	BIBLIOGRAPHIC DATA	LINK
Mouse mixed neuron-glia cerebellar cultures	Mouse	brain	unknown	adherent	plasmid	cotransfection (plasmid/plasmid)	K2 Transfection System	application note
Rat (Sprague-Dawley)	Rat	unknown	unknown		plasmid	in vivo application	Metafectene	publication	T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99
Mouse primary hippocampal neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426	Link
Spodoptera exigua hemocytes of late fourth instar larvae	Insect	unknown	unknown		plasmid	in vivo application	Metafectene PRO	application note
Plutella xylostella larval hemocoel (diamondback or cabbage moth)	Insect	unknown	unknown		plasmid		Metafectene PRO	publication	R. Ali et al., Comparative Biochem. and Physiol., 2015, Part A 179: 44-53; doi.org/10.1016/j.cbpa.2014.09.004
Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats)	Rat	muscle	primary cell	adherent	plasmid		K2 Transfection System	publication	E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113	Link
Rat primary pinealocyte cells	Rat	brain	primary cell		plasmid		Metafectene	publication	T.-D. Kim et al.,Genes & Dev., Apr 2007; 21: 797 - 810	Link
Neurosphere derived neuroprogenitor cells (isolated from newborn mice brains)	Mouse	brain	unknown	adherent	plasmid	cotransfection (plasmid/plasmid)	K2 Transfection System	publication	S.Y. Kim et al., J. of Cell Science, 2019, 132, jcs228940, doi:10.1242/jcs.228940	Link
Human cutaneous melanoma (FFPE tissue sections)	Human	skin	primary cell	adherent	plasmid		Metafectene PRO	publication	M. Venza et al., Biochim. et Biophys. Acta, 2015, 1849: 247-256; doi.org/10.1016/j.bbagrm.2014.12.004
Rat primary hepatocytes	Rat	digestive organs	primary cell	adherent	plasmid	cotransfection (plasmid/siRNA)	Metafectene PRO	publication	F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906
Mouse (BALB/c) primary alveolar macrophages	Mouse	immune system	primary cell		plasmid		Metafectene	publication	M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476	Link
Mouse cultured cortical neurons	Mouse	brain	primary cell	adherent	plasmid		Metafectene PRO	publication	A. Schneider, Dissertation, 2018, Albert-Ludwigs-Universität Freiburg
Tribolium castaneum (Hemocoel of late-instar larvae)	Insect	unknown	unknown		plasmid		Metafectene PRO	publication	R. Hepat et al., J. Virol., October 2013, Volume 87, Number 20, 11223–11230	Link
Rabitt (Lapine) articular chondrocytes	Rabbit	other	unknown		plasmid		Metafectene	publication	P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44
Mouse embryonic forebrain cultures	Mouse	brain	primary cell	adherent	plasmid		Metafectene	publication	S. Couillard-Despres et al., 2014, US 8,841,430	Link
Mouse hepatocytes	Mouse	digestive organs	primary cell	adherent	plasmid		Metafectene PRO	publication	S.-Y. Cai et al., JCI Insight. 2017, 2(5): e90780, doi.org/10.1172/jci.insight.90780
Rat ventricular myocytes	Rat	muscle	primary cell	adherent	plasmid	RNA-Interference	Metafectene	publication	B. Hu et al., BioScience Trends., 2012; 6(1): 26-32, DOI: 10.5582/bst.2012.v6.1.26	Link
Xenopus laevis (african clawed frog) intestinal fragments	Frog	digestive organs	unknown		plasmid		Metafectene EASY	application note
Rat primary granulosa cells isolated from Ovaries	Rat	genital tract	primary cell		plasmid		Metafectene	publication	J.-H. Kim et al., Nature Commun., 2014, 5:2936, DOI: 10.1038/ncomms3936	Link
Mouse fibroblast cell line, deficient of N-WASP	Mouse	unknown	cell line	adherent	plasmid		Metafectene	publication	S. Benesch et al., J. Cell Sci., Jul 2005; 118: 3103 - 3115	Link
Mouse articular chondrocytes	Mouse	other	primary cell	adherent	plasmid	cotransfection (plasmid/plasmid)	Metafectene	publication	J. Rhee, J. of Biol. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014	Link
Plutella xylostella larvae (diamondback or cabbage moth)	Insect	unknown	unknown		plasmid	in vivo application	Metafectene PRO	publication	W. Gad et al., J. Gen. Virol., Apr 2008; 89: 931 - 938	Link
Plutella xylostella hemocoel of late second instar larvae	Insect	unknown	unknown		plasmid	in vivo application	Metafectene PRO	publication	S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017
Mouse primary hepatocytes	Mouse	digestive organs	primary cell	adherent	plasmid		Metafectene PRO	publication	J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8
Rabbit (New Zealand) primary chondrocytes from articular cartilage	Rabbit	other	primary cell	adherent	plasmid		Metafectene	publication	Y. H. Huh et al., J. Biol. Chem., Jun 2007; 282: 17123 - 17131	Link

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