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Products Transfection
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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Rat (Sprague-Dawley) primary alveolar macrophages Rat immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

Human immortalized lymphocyte cell line (immortalized by EBV) Human immune system cell line suspension siRNA RNA-Interference Metafectene PRO publication M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065 Link

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

Mouse (BALB/c) primary alveolar macrophages Mouse immune system primary cell plasmid Metafectene publication M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 Link

2D8

chicken B cell line, reticuloendotheliosis virus transformed Chicken immune system cell line plasmid Metafectene PRO publication B. C. Viertlboeck et al., PNAS, Jul 2007; 104: 11718 - 11723 Link

2D8

chicken B cell line, reticuloendotheliosis virus transformed Chicken immune system cell line plasmid stable transfection Metafectene PRO publication V. Turowski et al., PLOSONE, DOI:10.1371/journal.pone.0151513 Link

2D8

chicken B cell line, reticuloendotheliosis virus transformed Chicken immune system cell line plasmid Metafectene PRO publication P. Petrov, Dissertation, 2016, University of Oulu Link

2D8

chicken B cell line, reticuloendotheliosis virus transformed Chicken immune system cell line plasmid Metafectene PRO publication P. Petrov et al., APMIS, 125: 106-113, DOI 10.1111/apm.12641

427.1.86

Mouse thymic nurse cells Mouse immune system unknown siRNA RNA-Interference Metafectene SI publication H. S. Lee et al., Biochem. and Biophys. Res. Commun., 2014, 452: 1084-1090, doi.org/10.1016/j.bbrc.2014.09.055

55.1

Human immortalized cord blood Human immune system cell line suspension plasmid Metafectene PRO application note

55.2

Human immortalized cord blood ATCC CRL-2155 Human immune system cell line suspension plasmid Metafectene PRO application note

CD4+ T cells

Human CD4+ T cells Human immune system suspension siRNA RNA-Interference Metafectene PRO publication J.-S. Chung et al., Eur. J. Immunol., 2011,41: 1794–1799, DOI 10.1002/eji.201041233

DG-75

Human Burkitt's lymphoma cell line ATCC CRL-2625 Human immune system cell line suspension plasmid Metafectene publication S. Thakker et al., J. Virol., May 2015; 89: 5536 - 5556

EL-4

Mouse lymphoma T cell line ATCC TIB-39 Mouse immune system cell line suspension plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication K. Ohl et al., Frontiers in Immunology, 2016, 7:618, doi: 10.3389/fimmu.2016.00618 Link

EL-4

Mouse T lymphoma cell line ATCC TIB-39 Mouse immune system cell line suspension plasmid Metafectene publication A. Sahoo et al., J. Biol. Chem., Oct 2008; 283: 28860 - 28872 Link

hADMC

Human primary adipose-derived mast cells Human immune system primary cell plasmid Metafectene PRO publication M. Motaghed et al., J. Immuntherapy of Cancer, 2021, 9(Suppl 2): A1–A1052, doi.org/10.1136/jitc-2021-SITC2021.220 Link

HD-11

Chicken leukaemic macrophage cell line Chicken immune system cell line adherent siRNA RNA-Interference Metafectene PRO publication H. Berberich et al., FEBS Open Bio, 2017, 7: 1909–1923, doi:10.1002/2211-5463.12323 Link

HECa10

Mouse endothelial cells of peripheral lymph nodes Mouse immune system cell line adherent plasmid CRISPR K2 Transfection System publication P. Janus et al., Cell Death & Differentiation, 2020, 27: 2280–2292, doi.org/10.1038/s41418-020-0501-8 Link

hHSC

Human hematopoietic stem cells (CD34+) Human immune system stem cell suspension plasmid K2 Transfection System application note

HL-60

Human adult acute myeloid leukemia cell line ATCC CCL-240 Human immune system cell line suspension siRNA RNA-Interference Metafectene PRO publication B. Landry et al., PLoS ONE, 2012, 7(8): e44197, doi:10.1371/journal.pone.0044197 Link

HL-60

Human adult acute myeloid leukemia cell line ATCC CCL-240 Human immune system cell line suspension plasmid Metafectene EASY publication J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona Link

hMDM

Human primary monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference Metafectene publication M. P. Morrow et al., Mol. Pharmacol., Aug 2010; 78: 215 - 225 Link

hMDM

Human monocyte-derived macrophages Human immune system unknown adherent plasmid Metafectene EASY publication J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona Link

hMDM

Human monocyte-derived macrophages Human immune system unknown adherent oligonucleotide K2 Transfection System application note

HPBL

Human primary peripheral blood lymphocytes Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

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