References
Products Transfection
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
| CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | A. D. Joshi et al., Mol. Pharmacol., Sept 2017, 92:366-374, doi.org/10.1124/mol.117.108878 | ||||
| Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice | Mouse | brain | primary cell | adherent | siRNA | Metafectene PRO | publication | M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126 | Link | ||||
| Rat sensory neurons isolated from dorsal root ganglia (DRG) | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 | Link | |||
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | J. S. Park et al., BMC Res Notes, Jan 2011; 4: 8 | ||||
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | A. D. Joshi et al., J. Pharmacol. Exp. Ther., Mar 2015; 353: 201 - 212 | ||||
| Rabbit (New Zealand) primary chondrocytes from articular cartilage | Rabbit | other | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047 | Link | |||
| Rat primary hepatocytes | Rat | digestive organs | primary cell | adherent | siRNA | cotransfection (plasmid/siRNA) | Metafectene PRO | publication | F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906 | ||||
| Human primary skin fibroblasts | Human | skin | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 | Link | |||
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental | ||||
| Mouse cultured fetal ovaries | Mouse | genital tract | primary cell | siRNA | RNA-Interference | Metafectene | publication | B. Xu et al., PLoS One, 2011, 6(1): e16046, doi: 10.1371/journal.pone.0016046 | Link | ||||
| 4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein | Mouse | unknown | unknown | siRNA | in vivo application | Metafectene | publication | S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 | Link | ||||
| Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) | Rat | muscle | primary cell | adherent | siRNA | RNA-Interference | K2 Transfection System | publication | E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 | Link | |||
| Rat (Wistar neonatal) primary hippocampal neurons | Rat | brain | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | P. J. Kammermeier, J. Neurosci., Aug 2008; 28: 8560 - 8567 | Link | |||
| Rat (Sprague Dawley young adult male) primary sensory neurons | Rat | nervous system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | M. R. Vasko et al., PLoS ONE, 2014, 9(8): e104529, doi:10.1371/journal.pone.0104529 | Link | |||
| Mouse (18.5 dpc fetal or neonatal) ovaries | Mouse | genital tract | unknown | siRNA | RNA-Interference | Metafectene | publication | H. Zhang et al., Reproduction, Jun 2014; 148: 43 - 54 | Link | ||||
| 4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein | Mouse | unknown | unknown | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 | Link | ||||
| Human primary chronic lymphocytic leukemia cells | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link | |||
| Human immortalized lymphocyte cell line (immortalized by EBV) | Human | immune system | cell line | suspension | siRNA | RNA-Interference | Metafectene PRO | publication | M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065 | Link | |||
| Mouse primary cortical cells | Mouse | brain | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | B.-H. Choi et al., J. Cell Sci., Apr 2006; 119: 1329 - 1340 | ||||
| Mouse podocytes | Mouse | urinary system | primary cell | adherent | siRNA | RNA-Interference | K2 Transfection System | publication | F. Kliewe et al., SCIENTIfIC REPORTS, nature.com, 7: 9916, DOI:10.1038/s41598-017-10116-4 | Link | |||
| Human primary fibroblasts | Human | unknown | primary cell | siRNA | RNA-Interference | Metafectene | publication | L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 | Link | ||||
| Mouse fetal ovaries | Mouse | genital tract | primary cell | siRNA | RNA-Interference | Metafectene PRO | publication | R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R. | Link | ||||
| Human primary fibroblasts (derived from ALS patients) | Human | unknown | cell line | adherent | siRNA | RNA-Interference | Metafectene | publication | M. Milani et al., J. of Neuroinflammation, 2021, 18: 132, doi.org/10.1186/s12974-021-02184-1 | Link | |||
| Mouse primary hepatocytes | Mouse | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | S. Surendran, PhD Thesis, 2014, Indiana University | ||||
| Rabbit (New Zealand) primary chondrocytes from articular cartilage | Rabbit | other | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | E.-H. Hong et al., J. Biol. Chem., Aug 2011; 286: 28619 - 28631 | Link |
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