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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Plutella xylostella, early third instar larvae (diamondback or cabbage moth) Insect unknown unknown plasmid in vivo application Metafectene PRO publication M. R. Ali et al., J. of Invertebrate Pathology, 2012, 110: 389–397, doi.org/10.1016/j.jip.2012.05.003

C57BL/6 mice bearing LLC Lewis lung carcinoma cells Mouse plasmid in vivo application Metafectene PRO publication V. Sankov et al., Prog. on Chem. and App. of Chitin and its Derivatives, 2017, 22: 190-200, DOI: 10.15259/PCACD.22.19 Link

Mouse cortical neuronal cells Mouse brain unknown adherent plasmid Metafectene EASY application note

Primary porcine granulosa cells Swine genital tract primary cell plasmid Metafectene PRO publication S-y Jung, Dissertation, 2020, Mississippi State University Link

Rat embryonic cerebral cortex Rat brain unknown plasmid Metafectene PRO application note

Plutella xylostella hemocoel of early third instar larvae (diamondback or cabbage moth) Insect unknown unknown plasmid in vivo application Metafectene PRO publication Y. Kim et al., Insect Science, 2016, 23: 235-244, DOI 10.1111/1744-7917.12237

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown plasmid in vivo application Metafectene PRO publication B. Park et al., Insect Science, 2012, 19: 47–54, DOI 10.1111/j.1744-7917.2011.01421.x

Rabbit articular chondrocytes Rabbit other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication J. Rhee, J. of Bio. Chem., 2012, 287, 15: 11751–11760, DOI 10.1074/jbc.M111.281014 Link

Mouse primary embryonic forebrain cells Mouse brain primary cell plasmid Metafectene publication C. Karl et al., J Neurochemistry, 2005, 92, 264-282

Mouse hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R.C. Weethmann et al., Neuropharmacology, 2020, doi.org/10.1016/j.neuropharm.2020.108426 Link

Mouse chondrocytes Mouse other primary cell adherent plasmid cotransfection (plasmid/plasmid) Metafectene publication A. Jash et al., PLoS ONE, 2012, 7(7): e40828, doi:10.1371/journal.pone.0040828 Link

Rat primary neonatal ventricular cardiomyocytes Rat muscle primary cell plasmid K2 Transfection System application note

Human fibrous dysplasia cells Human unknown unknown plasmid Metafectene publication P. Orth et al., Mol Biotechnol, Feb 2008; 38(2): 137-44

Human Praeadipocytes Human skin primary cell plasmid Metafectene publication G. Weisinger et al., US9663790B2 Link

Human primary hepatocytes Human digestive organs primary cell adherent plasmid RNA-Interference Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Human fibroblasts from CMT2B patients Human unknown primary cell adherent plasmid Metafectene PRO publication P. Saveri et al., Cells, 2020, 9, 1028, doi:10.3390/cells9041028 Link

Mouse immortalized skin fibroblasts (deficient of Lap2-alpha) Mouse unknown unknown adherent plasmid Metafectene PRO publication E. Bartova et al., Protoplasma, 2017, 254: 2035-2043, DOI 10.1007/s00709-017-1076-1

Plutella xylostella hemocoel of late second instar larvae Insect unknown unknown plasmid RNA-Interference Metafectene PRO publication S. Kumar et al., Comp. Biochem. and Physiol., 2014, Part A 177: 27-34, doi.org/10.1016/j.cbpa.2014.07.017

Chicken cerebellar granule neurons Chicken brain unknown adherent plasmid Metafectene PRO publication B. O. Strom et al., J Neurosci Methods, Oct 2010; 193(1): 39-46

Rabbit (New Zealand) primary chondrocytes from articular cartilage Rabbit other primary cell adherent plasmid Metafectene publication J.-H. Ryu et al., J. Biol. Chem., Aug 2006; 281: 22039 - 22047 Link

Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood Human other stem cell adherent plasmid K2 Transfection System publication S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681

Rat (Sprague-Dawley) Rat unknown unknown plasmid in vivo application Metafectene publication T. Holzbach et al., J Cell Mol Med, Mar 2010; 14(3): 587-99

Mouse primary hippocampal neurons Mouse brain primary cell adherent plasmid Metafectene PRO publication R. C.Werthmann et al., Neuropharmacology, 2021, 190: 108426, doi.org/10.1016/j.neuropharm.2020.108426 Link

Mouse mixed neuron-glia cerebellar cultures Mouse brain unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

Spodoptera exigua hemocytes of late fourth instar larvae Insect unknown unknown plasmid in vivo application Metafectene PRO application note

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