References
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
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Human immortalized lymphocyte cell line (immortalized by EBV) | Human | immune system | cell line | suspension | siRNA | RNA-Interference | Metafectene PRO | publication | M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065 | Link | |||
Human primary chronic lymphocytic leukemia cells | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link | |||
Mouse (BALB/c) primary alveolar macrophages | Mouse | immune system | primary cell | plasmid | Metafectene | publication | M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 | Link | |||||
Rat (Sprague-Dawley) primary alveolar macrophages | Rat | immune system | primary cell | plasmid | Metafectene | publication | M.-Y. Kim et al., J. Cell Sci., May 2008; 121: 1466 - 1476 | Link | |||||
2D8 |
chicken B cell line, reticuloendotheliosis virus transformed | Chicken | immune system | cell line | plasmid | Metafectene PRO | publication | P. Petrov, Dissertation, 2016, University of Oulu | Link | ||||
2D8 |
chicken B cell line, reticuloendotheliosis virus transformed | Chicken | immune system | cell line | plasmid | Metafectene PRO | publication | P. Petrov et al., APMIS, 125: 106-113, DOI 10.1111/apm.12641 | |||||
2D8 |
chicken B cell line, reticuloendotheliosis virus transformed | Chicken | immune system | cell line | plasmid | Metafectene PRO | publication | B. C. Viertlboeck et al., PNAS, Jul 2007; 104: 11718 - 11723 | Link | ||||
2D8 |
chicken B cell line, reticuloendotheliosis virus transformed | Chicken | immune system | cell line | plasmid | stable transfection | Metafectene PRO | publication | V. Turowski et al., PLOSONE, DOI:10.1371/journal.pone.0151513 | Link | |||
427.1.86 |
Mouse thymic nurse cells | Mouse | immune system | unknown | siRNA | RNA-Interference | Metafectene SI | publication | H. S. Lee et al., Biochem. and Biophys. Res. Commun., 2014, 452: 1084-1090, doi.org/10.1016/j.bbrc.2014.09.055 | ||||
55.1 |
Human immortalized cord blood | Human | immune system | cell line | suspension | plasmid | Metafectene PRO | application note | ![]() |
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55.2 |
Human immortalized cord blood | ATCC CRL-2155 | Human | immune system | cell line | suspension | plasmid | Metafectene PRO | application note | ![]() |
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CD4+ T cells |
Human CD4+ T cells | Human | immune system | suspension | siRNA | RNA-Interference | Metafectene PRO | publication | J.-S. Chung et al., Eur. J. Immunol., 2011,41: 1794–1799, DOI 10.1002/eji.201041233 | ||||
DG-75 |
Human Burkitt's lymphoma cell line | ATCC CRL-2625 | Human | immune system | cell line | suspension | plasmid | Metafectene | publication | S. Thakker et al., J. Virol., May 2015; 89: 5536 - 5556 | |||
EL-4 |
Mouse lymphoma T cell line | ATCC TIB-39 | Mouse | immune system | cell line | suspension | plasmid | cotransfection (plasmid/plasmid) | K2 Transfection System | publication | K. Ohl et al., Frontiers in Immunology, 2016, 7:618, doi: 10.3389/fimmu.2016.00618 | Link | |
EL-4 |
Mouse T lymphoma cell line | ATCC TIB-39 | Mouse | immune system | cell line | suspension | plasmid | Metafectene | publication | A. Sahoo et al., J. Biol. Chem., Oct 2008; 283: 28860 - 28872 | Link | ||
hADMC |
Human primary adipose-derived mast cells | Human | immune system | primary cell | plasmid | Metafectene PRO | publication | M. Motaghed et al., J. Immuntherapy of Cancer, 2021, 9(Suppl 2): A1–A1052, doi.org/10.1136/jitc-2021-SITC2021.220 | Link | ||||
HD-11 |
Chicken leukaemic macrophage cell line | Chicken | immune system | cell line | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | H. Berberich et al., FEBS Open Bio, 2017, 7: 1909–1923, doi:10.1002/2211-5463.12323 | Link | ||
HECa10 |
Mouse endothelial cells of peripheral lymph nodes | Mouse | immune system | cell line | adherent | plasmid | CRISPR | K2 Transfection System | publication | P. Janus et al., Cell Death & Differentiation, 2020, 27: 2280–2292, doi.org/10.1038/s41418-020-0501-8 | Link | ||
hHSC |
Human hematopoietic stem cells (CD34+) | Human | immune system | stem cell | suspension | plasmid | K2 Transfection System | application note | ![]() |
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HL-60 |
Human adult acute myeloid leukemia cell line | ATCC CCL-240 | Human | immune system | cell line | suspension | siRNA | RNA-Interference | Metafectene PRO | publication | B. Landry et al., PLoS ONE, 2012, 7(8): e44197, doi:10.1371/journal.pone.0044197 | Link | |
HL-60 |
Human adult acute myeloid leukemia cell line | ATCC CCL-240 | Human | immune system | cell line | suspension | plasmid | Metafectene EASY | publication | J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona | Link | ||
hMDM |
Human monocyte-derived macrophages | Human | immune system | unknown | adherent | plasmid | Metafectene EASY | publication | J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona | Link | |||
hMDM |
Human monocyte-derived macrophages | Human | immune system | unknown | adherent | oligonucleotide | K2 Transfection System | application note | ![]() |
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hMDM |
Human primary monocyte-derived macrophages | Human | immune system | primary cell | siRNA | RNA-Interference | Metafectene | publication | M. P. Morrow et al., Mol. Pharmacol., Aug 2010; 78: 215 - 225 | Link | |||
HPBL |
Human primary peripheral blood lymphocytes | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link |