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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

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If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Human tenon’s capsule fibroblasts Human sensory organs unknown oligonucleotide Metafectene publication H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290

Mouse retinal ganglion cells (from multipotent retinal stem cells) Mouse sensory organs stem cell adherent plasmid K2 Transfection System publication D.-W. Chen et al., Prec. Nanomed., 2018, 1(2):106-123, DOI: 10.29016/180711.1 Link

Mouse (C57Bl/L6) cells in the area of the organ of Corti Mouse sensory organs unknown plasmid in vivo application Metafectene publication M. Praetorius et al., HNO, May 2008; 56(5): 524-9

Human primary pterygium fibroblasts Human sensory organs primary cell adherent plasmid RNA-Interference Metafectene PRO publication Y. Su et al., Mol. Vis., 2011, 17: 247–256 Link

Mouse (BALB/c mice) organ of Corti explants Mouse sensory organs unknown miRNA K2 Transfection System publication Y. Li et al., Cell Death and Disease, 2016, 7:e2362, doi:10.1038/cddis.2016.246 Link

A7

Rat transformed retinal astrcyte cell line Rat sensory organs cell line adherent plasmid K2 Transfection System application note

A7

Rat transformed retinal astrcyte cell line Rat sensory organs cell line adherent plasmid K2 Transfection System publication D. W. Chen et al., Drug Deliv. and Transl. Res., DOI 10.1007/s13346-016-0324-9

ARPE-19

Human ephitelial retina cells ATCC CRL-2302 Human sensory organs unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

ARPE-19

Human ephitelial retina cells ATCC CRL-2302 Human sensory organs unknown adherent plasmid K4 Transfection System application note

ARPE-19

Human ephitelial retina cells ATCC CRL-2302 Human sensory organs unknown adherent plasmid cotransfection (plasmid/plasmid) K2 Transfection System application note

hConEC

Human primary conjunctival epithel cells Human sensory organs primary cell adherent miRNA Metafectene SI publication Q. Pilson et al., Scientific Reports, 2020, 10: 7484, doi.org/10.1038/s41598-020-64422-5 Link

hESC-RPE

Human embryonic stem cell-derived retinal pigment epithelium cells Human sensory organs primary cell siRNA RNA-Interference Metafectene PRO publication E. Ramsay et al., Pharmaceutics, 2020, 12(7): 667, doi: 10.3390/pharmaceutics12070667 Link

hLEC

Human lens epithelial cell line Human sensory organs cell line adherent plasmid RNA-Interference Metafectene PRO publication Y. Su et al., Molecular Vision, 2012, 18: 601-605 Link

hLEC

Human lens epithelial cell line Human sensory organs cell line adherent plasmid stable transfection Metafectene PRO publication Y. Su et al., Molecular Vision, 2012, 18: 601-605 Link

hOMs

Human primary olfactory mucosal cells Human sensory organs primary cell adherent siRNA RNA-Interference Metafectene SI publication S. Chew et al., Chew et al. Particle and Fibre Toxicology , 2020, 17: 18, doi.org/10.1186/s12989-020-00352-4 Link

hRPE

Human retinal pigment epithelium cells Human sensory organs unknown plasmid Metafectene PRO publication I. Knütter et al., J. Pharmacol. Exp. Ther., Nov 2008; 327: 432 - 441 Link

hRPEC

Human retinal pigment epithelial cells Human sensory organs primary cell adherent siRNA stable transfection Metafectene publication I-Ta Lee et al., PLoS ONE 10(2): e0117911. doi:10.1371/journal.pone.0117911 Link

hTM

Human trabecular meshwork cells Human sensory organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication Y. Su et al., Molecular Vision, 2012, 18: 1881-1884, Link

MiEye8 retinal 3D neurospheres

Mouse retinal 3D neurospheres (from multipotent retinal stem cells) Mouse sensory organs stem cell plasmid 3D cell culture K2 Transfection System publication D.-W. Chen et al., Prec. Nanomed., 2018, 1(2):106-123, DOI: 10.29016/180711.1 Link

NTM

Human normal trabecular meshwork cells Human sensory organs primary cell adherent plasmid K2 Transfection System publication T. Carreon, University of Miami, Scholarly Repository, Open Access Dissertations Link

NTM

Human normal trabecular meshwork cells Human sensory organs primary cell adherent plasmid K2 Transfection System publication T. A. Carreon et al., SCIENTIfIC REPORTS, nature.com, 7:452, DOI:10.1038/s41598-017-00430-2

NTM153-00

Human primary trabecular meshwork cells Human sensory organs primary cell adherent plasmid Metafectene EASY application note

NTM174-04

Human primary trabecular meshwork cells Human sensory organs primary cell adherent plasmid Metafectene EASY application note

NTM210-05

Human primary trabecular meshwork cells Human sensory organs primary cell adherent plasmid Metafectene EASY application note

NTM486-04

Human primary trabecular meshwork cells Human sensory organs primary cell adherent plasmid Metafectene EASY application note

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