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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Human keratinocytes isolated from neonatal foreskin Human skin primary cell adherent siRNA RNA-Interference Metafectene publication S. Bruche, PhD Thesis, 2014, Imperial College London Link

Human primary fibroblasts Human unknown primary cell siRNA RNA-Interference Metafectene publication L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 Link

Human primary hepatocytes Human digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 Link

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

Human peripheral blood mononuclear cell (PBMC)-derived macrophages Human unknown primary cell adherent siRNA RNA-Interference K2 Transfection System publication T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8 Link

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360 Link

Human primary skin fibroblasts Human skin primary cell adherent siRNA RNA-Interference Metafectene publication M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 Link

1BR.3

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication D. Alcantara, PhD Thesis, 2012, University of Sussex Link

1BR.3

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO publication I. Abramowicz et al., Hum. Mol. Genet., Dec 2016, DOI: 10.1093/hmg/ddw364

1BR3

Human primary fibroblasts Human unknown primary cell adherent siRNA RNA-Interference Metafectene PRO application note

48BR

Human primary fibroblasts Human unknown primary cell siRNA RNA-Interference Metafectene PRO publication H. Brunton et al., Mol. Cell. Biol., Oct 2011; 31: 4022 - 4035 Link

CAF

Human carcinoma associated fibroblasts Human digestive organs primary cell adherent siRNA RNA-Interference Metafectene publication A. Min et al., J. Biochem. Molecular Toxicology, 2015, 29(2): 70-76; DOI 10.1002/jbt

CMel-2

Human primary superficial spreading melanoma cells Human skin primary cell adherent siRNA RNA-Interference Metafectene PRO publication I. Venza et al., Int. Immunopharmacol., 2014, 21: 439–446; doi.org/10.1016/j.intimp.2014.05.024

hBMSC

Human bone marrow-derived mesenchymal stem cells Human bone primary cell adherent siRNA RNA-Interference Metafectene PRO publication E. J. Lee et al., Mol. Therapy, 2017, 26(3): 845-859, doi.org/10.1016/j.ymthe.2017.12.015 Link

hESC-RPE

Human embryonic stem cell-derived retinal pigment epithelium cells Human sensory organs primary cell siRNA RNA-Interference Metafectene PRO publication E. Ramsay et al., Pharmaceutics, 2020, 12(7): 667, doi: 10.3390/pharmaceutics12070667 Link

HGF

Human gingival fibroblasts Human digestive organs primary cell adherent siRNA RNA-Interference Metafectene publication A. Min et al., J. Biochem. Molecular Toxicology, 2015, 29(2): 70-76; DOI 10.1002/jbt

HLF

Human lung fibroblasts Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene PRO publication X. Deng et al., J. Cell. Biochem., 2017, 118: 4012-4019, DOI 10.1002/jcb.26057

HMC

Human mesangial cells Human unknown primary cell adherent siRNA RNA-Interference Metafectene publication J. L. Cano-Penalver et al., Mol. Med., 2015, 21:873-885; doi: 10.2119/molmed.2015.00059 Link

hMDM

Human primary monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference Metafectene publication M. P. Morrow et al., Mol. Pharmacol., Aug 2010; 78: 215 - 225 Link

hOMs

Human primary olfactory mucosal cells Human sensory organs primary cell adherent siRNA RNA-Interference Metafectene SI publication S. Chew et al., Chew et al. Particle and Fibre Toxicology , 2020, 17: 18, doi.org/10.1186/s12989-020-00352-4 Link

hPAEpiC

Human pulmonary alveolar epithelial cells Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene publication C.-C. Lin, PLoS ONE, 2015, 10(3): e0118473; doi:10.1371/journal.pone.0118473 Link

HPAEpiC

Human pulmonary alveolar epithelial cells Human respiratory system primary cell adherent siRNA RNA-Interference Metafectene publication C.-C. Lin et al., Front. Pharmacol. 2016, 7:80; doi: 10.3389/fphar.2016.00080 Link

hPASMC

Human pulmonary artery smooth muscle cells Human respiratory system primary cell adherent siRNA RNA-Interference K2 Transfection System publication Z. Qian et al., Am. J. Physiol. Cell Physiol., July 2017, 313: C380 -C391; doi:10.1152/ajpcell.00061.2017

HPBL

Human primary peripheral blood lymphocytes Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

hPBMC

Human peripheral blood monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference K2 Transfection System publication D. Pires et al., Scientific Reports, 2016, 6:32247, DOI: 10.1038/srep32247 Link

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