References
Products Transfection
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Instruction for Use:
In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.
No reference? > General Selection Guide
If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.
| CELL TYPE | DESCRIPTION OF CELL TYPE | CAT.NO. | SPECIES | ORIGIN OF CELLS | COMMON CELL TYPE | GROWTH PROPERTIES | NUCLEIC ACID / DELIVERED MOLECULE | APPLICATION | PRODUCT | REFERENCE TYPE | BIBLIOGRAPHIC DATA | LINK | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Human keratinocytes isolated from neonatal foreskin | Human | skin | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | S. Bruche, PhD Thesis, 2014, Imperial College London | Link | |||
| Human primary fibroblasts | Human | unknown | primary cell | siRNA | RNA-Interference | Metafectene | publication | L. Woodbine et al., Nucleic Acids Res., Sep 2011; 39: 6986 - 6997 | Link | ||||
| Human primary hepatocytes | Human | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | J.-S. Park et al., BMC Res. Notes, 2011, 4: 8, doi:10.1186/1756-0500-4-8 | Link | |||
| Human primary chronic lymphocytic leukemia cells | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link | |||
| Human peripheral blood mononuclear cell (PBMC)-derived macrophages | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | K2 Transfection System | publication | T. Troiani et al., British Journal of Cancer, 2020, 122: 1782–1790, doi.org/10.1038/s41416-020-0840-8 | Link | |||
| Human primary fibroblasts | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | T. Stiff et al., PLoS Genet., 2013, 9(3): e1003360, doi.org/10.1371/journal.pgen.1003360 | Link | |||
| Human primary skin fibroblasts | Human | skin | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | M. Milani et al., ResearchSquare, 2021, doi.org/10.21203/rs.3.rs-138253/v1 | Link | |||
1BR.3 |
Human primary fibroblasts | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | D. Alcantara, PhD Thesis, 2012, University of Sussex | Link | ||
1BR.3 |
Human primary fibroblasts | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | I. Abramowicz et al., Hum. Mol. Genet., Dec 2016, DOI: 10.1093/hmg/ddw364 | |||
1BR3 |
Human primary fibroblasts | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | application note | |
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48BR |
Human primary fibroblasts | Human | unknown | primary cell | siRNA | RNA-Interference | Metafectene PRO | publication | H. Brunton et al., Mol. Cell. Biol., Oct 2011; 31: 4022 - 4035 | Link | |||
CAF |
Human carcinoma associated fibroblasts | Human | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | A. Min et al., J. Biochem. Molecular Toxicology, 2015, 29(2): 70-76; DOI 10.1002/jbt | |||
CMel-2 |
Human primary superficial spreading melanoma cells | Human | skin | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | I. Venza et al., Int. Immunopharmacol., 2014, 21: 439–446; doi.org/10.1016/j.intimp.2014.05.024 | |||
hBMSC |
Human bone marrow-derived mesenchymal stem cells | Human | bone | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | E. J. Lee et al., Mol. Therapy, 2017, 26(3): 845-859, doi.org/10.1016/j.ymthe.2017.12.015 | Link | ||
hESC-RPE |
Human embryonic stem cell-derived retinal pigment epithelium cells | Human | sensory organs | primary cell | siRNA | RNA-Interference | Metafectene PRO | publication | E. Ramsay et al., Pharmaceutics, 2020, 12(7): 667, doi: 10.3390/pharmaceutics12070667 | Link | |||
HGF |
Human gingival fibroblasts | Human | digestive organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | A. Min et al., J. Biochem. Molecular Toxicology, 2015, 29(2): 70-76; DOI 10.1002/jbt | |||
HLF |
Human lung fibroblasts | Human | respiratory system | primary cell | adherent | siRNA | RNA-Interference | Metafectene PRO | publication | X. Deng et al., J. Cell. Biochem., 2017, 118: 4012-4019, DOI 10.1002/jcb.26057 | |||
HMC |
Human mesangial cells | Human | unknown | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | J. L. Cano-Penalver et al., Mol. Med., 2015, 21:873-885; doi: 10.2119/molmed.2015.00059 | Link | ||
hMDM |
Human primary monocyte-derived macrophages | Human | immune system | primary cell | siRNA | RNA-Interference | Metafectene | publication | M. P. Morrow et al., Mol. Pharmacol., Aug 2010; 78: 215 - 225 | Link | |||
hOMs |
Human primary olfactory mucosal cells | Human | sensory organs | primary cell | adherent | siRNA | RNA-Interference | Metafectene SI | publication | S. Chew et al., Chew et al. Particle and Fibre Toxicology , 2020, 17: 18, doi.org/10.1186/s12989-020-00352-4 | Link | ||
hPAEpiC |
Human pulmonary alveolar epithelial cells | Human | respiratory system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | C.-C. Lin, PLoS ONE, 2015, 10(3): e0118473; doi:10.1371/journal.pone.0118473 | Link | ||
HPAEpiC |
Human pulmonary alveolar epithelial cells | Human | respiratory system | primary cell | adherent | siRNA | RNA-Interference | Metafectene | publication | C.-C. Lin et al., Front. Pharmacol. 2016, 7:80; doi: 10.3389/fphar.2016.00080 | Link | ||
hPASMC |
Human pulmonary artery smooth muscle cells | Human | respiratory system | primary cell | adherent | siRNA | RNA-Interference | K2 Transfection System | publication | Z. Qian et al., Am. J. Physiol. Cell Physiol., July 2017, 313: C380 -C391; doi:10.1152/ajpcell.00061.2017 | |||
HPBL |
Human primary peripheral blood lymphocytes | Human | immune system | primary cell | suspension | siRNA | RNA-Interference | Metafectene | publication | S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 | Link | ||
hPBMC |
Human peripheral blood monocyte-derived macrophages | Human | immune system | primary cell | siRNA | RNA-Interference | K2 Transfection System | publication | D. Pires et al., Scientific Reports, 2016, 6:32247, DOI: 10.1038/srep32247 | Link |









