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Reference type
  1. application note (11)
  2. publication (49)
Common cell type
  1. cell line (46)
  2. primary cell (8)
  3. stem cell (1)
  4. unknown (4)
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  1. plasmid (36)
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  1. RNA-Interference (19)
  2. cotransfection (plasmid/plasmid) (3)
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  1. Metafectene (24)
  2. Metafectene EASY (6)
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  4. Metafectene SI (1)
  5. K2 Transfection System (4)
  6. DOTAP (4)

References

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Products Transfection
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Instruction for Use:

In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Human immortalized lymphocyte cell line (immortalized by EBV) Human immune system cell line suspension siRNA RNA-Interference Metafectene PRO publication M. Pradas-Juni et al., Mol. Endocrinoly, 2014, 28(9): 1558-1570, doi: 10.1210/me.2014-1065 Link

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

55.1

 Human immortalized cord blood Human immune system cell line suspension plasmid Metafectene PRO application note

55.2

 Human immortalized cord blood ATCC CRL-2155 Human immune system cell line suspension plasmid Metafectene PRO application note

CD4+ T cells

 Human CD4+ T cells Human immune system suspension siRNA RNA-Interference Metafectene PRO publication J.-S. Chung et al., Eur. J. Immunol., 2011,41: 1794–1799, DOI 10.1002/eji.201041233

DG-75

 Human Burkitt's lymphoma cell line ATCC CRL-2625 Human immune system cell line suspension plasmid Metafectene publication S. Thakker et al., J. Virol., May 2015; 89: 5536 - 5556

hADMC

 Human primary adipose-derived mast cells Human immune system primary cell plasmid Metafectene PRO publication M. Motaghed et al., J. Immuntherapy of Cancer, 2021, 9(Suppl 2): A1–A1052, doi.org/10.1136/jitc-2021-SITC2021.220 Link

hHSC

 Human hematopoietic stem cells (CD34+) Human immune system stem cell suspension plasmid K2 Transfection System application note

HL-60

 Human adult acute myeloid leukemia cell line ATCC CCL-240 Human immune system cell line suspension plasmid Metafectene EASY publication J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona Link

HL-60

 Human adult acute myeloid leukemia cell line ATCC CCL-240 Human immune system cell line suspension siRNA RNA-Interference Metafectene PRO publication B. Landry et al., PLoS ONE, 2012, 7(8): e44197, doi:10.1371/journal.pone.0044197 Link

hMDM

 Human monocyte-derived macrophages Human immune system unknown adherent oligonucleotide K2 Transfection System application note

hMDM

 Human monocyte-derived macrophages Human immune system unknown adherent plasmid Metafectene EASY publication J. C. Romero Sandoval, PhD Thesis, 2014, Universitat de Barcelona Link

hMDM

 Human primary monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference Metafectene publication M. P. Morrow et al., Mol. Pharmacol., Aug 2010; 78: 215 - 225 Link

HPBL

 Human primary peripheral blood lymphocytes Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

hPBMC

 Human primary peripheral blood mononuclear cells Human immune system primary cell suspension plasmid Metafectene publication S. Smith, PhD Thesis, 2013, Royal College of Surgeons in Ireland Link

hPBMC

 Human primary peripheral blood mononuclear cells Human immune system primary cell suspension plasmid Metafectene SI publication S. Smith, PhD Thesis, 2013, Royal College of Surgeons in Ireland Link

hPBMC

 Human peripheral blood monocyte-derived macrophages Human immune system primary cell siRNA RNA-Interference K2 Transfection System publication D. Pires et al., Scientific Reports, 2016, 6:32247, DOI: 10.1038/srep32247 Link

iDC

 Human immature dentritic cells Human immune system primary cell plasmid Metafectene publication Z. K. Nazarkina et al., Mol. Biology, 2018, 52(2): 222–231, DOI: 10.1134/S0026893317050132

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid Metafectene PRO publication V. Witte et al., J. Immunol., Dec 2008; 181: 8425 - 8432 Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid cotransfection (plasmid/plasmid) K2 Transfection System publication K. Marek-Bukowiec et al., Genes and Immunity, 2016, 17: 313-320, doi:10.1038/gene.2016.25 Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid Metafectene publication Q. Chen et al., J. Biol. Chem., Jul 2006; 281: 19985 - 19994 Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid Metafectene PRO application note

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid Metafectene PRO publication A. K. V. Iyer et al., J. Immunol., 2011, 187(6): 3256–3266, doi:10.4049/jimmunol.1002915. Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension plasmid Metafectene PRO publication A. K. V. Iyer et al., J. Immunol., Sep 2011; 187: 3256 - 3266 Link

Jurkat

 Human leukemia T-cell line ECACC 88042803 Human immune system cell line suspension oligonucleotide DOTAP publication A. Avinó et al., Protocols in Nucleic Acid Chemistry, 2020, 77: e78, doi: 10.1002/cpnc.78

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