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In the „Transfection Reagent Selection Guide“ you can find existing references by setting different filters e.g. for your combination of nucleic acid and cell type. Appropriate keywords in the “Search references“ at the top of the page can also yield a result. A reference indicates that the found transfection reagent has been successfully used for the corresponding application. Ideally, tested transfection parameters and transfection efficiencies are specified.

No reference? > General Selection Guide

If the list does not include a reference concerning your cell type, this does not mean that there is no suitable reagent available, but only that there is no report of experiences with this cell type. In this case it is necessary to preselect (General Selection Guide) and test a reagent (Free Sample Offer). If you want to write an application note for us we will make you an Offer for consideration.



CELL TYPE DESCRIPTION OF CELL TYPE CAT.NO. SPECIES ORIGIN OF CELLS COMMON CELL TYPE GROWTH PROPERTIES NUCLEIC ACID / DELIVERED MOLECULE APPLICATION PRODUCT REFERENCE TYPE BIBLIOGRAPHIC DATA PDF LINK

Plutella xylostella, third instar larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Z. Wang et al., PLoS Genet., 2021, 17(9): e1009751. https://doi.org/10.1371/journal.pgen.1009751 Link

Rat primary hepatocytes Rat digestive organs primary cell adherent siRNA cotransfection (plasmid/siRNA) Metafectene PRO publication F. Damiano et al., Biochem. J., 2013, 449: 543–553, doi:10.1042/BJ20120906

Chicken cerebellar granule neurons Chicken brain primary cell adherent plasmid Metafectene PRO publication L. P. E. Austdal et al., Journal of Neuroendocrinology, 2016, 28, doi: 10.1111/jne.1243

Rat primary hepatocytes (Sprague Dawley rats) Rat digestive organs primary cell adherent siRNA RNA-Interference Metafectene PRO publication R. Jog et al., Physiol. Reports., 2021, 9: e14991, DOI: 10.14814/phy2.14991 Link

Rat (Sprague Dawley young adult male) primary sensory neurons Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication Y. H. Zhang et al., J Neurophysiol, Jan 2012; 107: 315 - 335 Link

Rat primary neonatal cardiomyocytes (isolated from the heart of 18-day-old embryos of Sprague-Dawley rats) Rat muscle primary cell adherent plasmid K2 Transfection System publication E. Echeverría et al., Front. Pharmacol., 2020, 11: 113, doi: 10.3389/fphar.2020.00113 Link

Maruca vitrata late L4 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. Al Baki et al., Pest Manag. Sci., 2020, 76: 1020–1030, DOI: 10.1002/ps.5612 Link

Plutella xylostella larvae (diamondback or cabbage moth) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication Y. Park et al., Insect Molecular Biology, 2015, 24(6): 624-633; doi: 10.1111/imb.12188

Rat sensory neurons isolated from dorsal root ganglia (DRG) Rat nervous system primary cell adherent siRNA RNA-Interference Metafectene publication C. Li et al., J. of Neuroinflammation, 2015, DOI 10.1186/s12974-015-0286-8 Link

4-6-week old Balb/c IL2 NOD SCID (null) mice injected into tail vein Mouse unknown unknown siRNA RNA-Interference Metafectene publication S. Romano et al., Cell Death and Disease, 2013, 4: e578, doi:10.1038/cddis.2013.109 Link

Human non-embryonic CD34+multipotent stem cells isolated from umbilical cord blood Human other stem cell adherent plasmid K2 Transfection System publication S. Sharma et al., Cell Biol. Int., 2016, 40: 1366-1371, doi: 10.1002/cbin.10681

Mouse primary brown adipocyte Mouse other primary cell siRNA RNA-Interference K2 Transfection System publication J. M. Olsen et al., J. Cell Biol., Nov 2014; 207: 365 - 374 Link

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication A. Al Baki et al., Arch. Insect Biochem. Physiol., 2019, 100: e21524, DOI: 10.1002/arch.21524 Link

Mouse cerebral cortical tissue (astrocytes) of 2-day-old SAMR1 and SAMP8 mice Mouse brain primary cell adherent siRNA Metafectene PRO publication M .J. Alvarez-López et al., J. of Neuroinflammation, 2014, 11:126, doi:10.1186/1742-2094-11-126 Link

Human tenon’s capsule fibroblasts Human sensory organs unknown oligonucleotide Metafectene publication H. Mietz et al., Invest. Ophthalmol. Vis. Sci., May 2003; 44: 4290

Mouse fetal ovaries Mouse genital tract primary cell siRNA RNA-Interference Metafectene PRO publication R. Rosario et al., FASEB J., 2019, 33(12): 14221-14233, doi: 10.1096/fj.201901247R. Link

Mouse primary hepatocytes Mouse digestive organs primary cell adherent miRNA Metafectene PRO publication C. Castano et al., PNAS, 2020, 117, 48: 30335–30343, doi:10.1073/pnas.2016112117/-/DCSupplemental

Maruca vitrata hemocoel of L5 larvae (legume pod borer) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication A. A. Baki et al., Arch. Insect Biochem. Physiol., 2019:100:e21524, doi.org/10.1002/arch.21524

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication M. Vatanparast et al., Dev. and Comp. Immunology, 2019, 95: 108–117, doi.org/10.1016/j.dci.2019.02.008 Link

Spodoptera exigua fifth instar larvae (L5) (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication S. Ahmed et al., Front. Physiol., 2018, 9:1231, doi: 10.3389/fphys.2018.01231 Link

Human primary chronic lymphocytic leukemia cells Human immune system primary cell suspension siRNA RNA-Interference Metafectene publication S. Romano et al., Haematologica, Jul 2008; 93: 1039 - 1048 Link

Spodoptera exigua larvae (L5) (beet armyworm) Insect unknown unknown dsRNA in vivo application Metafectene PRO publication M. C. Roy et al., Front. Immunol., 2021, 2: 791319, doi: 10.3389/fimmu.2021.791319 Link

Spodoptera exigua adult larvae (beet armyworm) Insect unknown unknown dsRNA RNA-Interference Metafectene PRO publication S. Ahmed et al., Arch. Insect Biochem. Physiol.,2021, 106: e21748, doi.org/10.1002/arch.21748 Link

Hamster fetal ovary (organ culture) Hamster genital tract organ siRNA RNA-Interference Metafectene publication S. K. Roy et al., Methods and Protocols, 825, DOI 10.1007/978-1-61779-436-0_12

Mouse cortical neuronal cells Mouse brain unknown adherent plasmid Metafectene EASY application note

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